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Lab 6b - Analysis of Lambda DNA

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Analysis of Eco RI Cleavage Patterns of Lambda DNA

This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.

Experiments Include:, Complete instructions, Ready-to-load(tm) Predigested DNA samples, UltraSpec-Agarose(tm) powder, Practice Gel Loading Solution, Electrophoresis buffer (50x), InstaStain(tm) Methylene Blue, Methylene Blue Plus(tm), 1 ml Pipet, 100 ml Graduated Cylinder, and Microtipped transfer pipets. For 6 Gels - 3 lanes each.

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